The dry blood spot (DBS) kit offers a method to collect blood in order to undertake a diagnostic test in patients that are suspected of suffering from Gaucher disease. It is important that the clinical diagnosis is confirmed by a combination of genetic and other biomarker testing specific to the disease, and helps to ensure that patients receive timely specialist support and treatment.
The DBS kit should be used following the initial identification of symptoms that may be suggestive of Gaucher disease.
DBS Kit Contents
One DBS test request form attached to a filter card for collection of blood spots
The patient informed consent form
One pre-addressed ‘return to lab’ envelope
Blood can be taken either by venipucture or by pricking a finger
Disinfect the patient’s arm or finger
Preform venipuncture following EDTA procedures from the arm or, puncture the selected finger using a sterile lancet
Blood is collected onto DBS cards, processed and sent for testing
Pipette 50 μl of required sample or approximately 3 drops from the finger onto the circle without touching the surface of the filter card. The entire circle should be uniformly saturated. When correctly applied, the blood spot will be visible on the back of the card.
Continue procedure until the 10 required blood spots are completely saturated. The card should then be air dried for at least 3 hours. It is recommended that completed filter cards are sent within 1-2 weeks.